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Title: Quantifying the Mechanical Micro-environment during Three-dimensional Cell Expansion on Microbeads by means of Individual Cell-based Modelling
Authors: Smeets, Bart ×
Odenthal, Tim
Tijskens, Engelbert
Ramon, Herman
Van Oosterwyck, Hans #
Issue Date: 2013
Publisher: Taylor & Francis
Series Title: Computer Methods in Biomechanics and Biomedical Engineering vol:16 issue:10 pages:1071-1084
Abstract: Controlled in vitro three-dimensional cell expansion requires culture conditions that op-
timize the biophysical micro-environment of the cells during proliferation. In this study we
propose an individual cell-based modelling platform for simulating the mechanics of cell ex-
pansion on microcarriers. The lattice-free, particle-based method considers cells as individual
interacting particles that deform and move over time. The model quantifies how the mechani-
cal micro-environment of individual cells changes during the time of confluency. A sensitivity
analysis is performed, which shows that changes in the cell-specific properties of cell-cell
adhesion and cell stiffness cause the strongest change in the mechanical micro-environment
of the cells. Furthermore, the influence of the mechanical properties of cells and microbead
is characterized. The mechanical micro-environment is strongly influenced by the adhesive
properties and the size of the microbead. Simulations show that even in the absence of strong
biological heterogeneity a large heterogeneity in mechanical stresses can be expected purely
due to geometric properties of the culture system.
ISSN: 1025-5842
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
Mechanical Engineering - miscellaneous
Biomechanics Section
× corresponding author
# (joint) last author

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