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Title: CHARACTERIZING THE MECHANICAL MICROENVIRONMENT OF 3D CELL CULTURES USING INDIVIDUAL-BASED MODELS
Authors: Smeets, Bart
Odenthal, Tim
Van Oosterwyck, Hans
Ramon, Herman
Issue Date: 11-Apr-2012
Host Document: The Proceedings of the 10th International Symposium on Computer Methods in Biomechanics and Biomedical Engineering pages:135-141
Conference: Computer Methods in Biomechanics and Biomedical Engineering edition:10 location:Hotel Berlin, Berlin, Germany date:7-11 April 2012
Abstract: The influence of the mechanical microenvironment on proliferation of progenitor cells in 3D suspension cultures is still poorly understood. We developed a software framework for individual based modeling of three-dimensional cell cultures. The model simulates the proliferation of progenitor cells on microcarriers. The mechanics of the cells are modeled by the Johnson-Kendall-
Roberts potential and the equations of motion of the cells are derived for a friction-dominated system. The cell cycle is broken up into two stages: increase of cell volume and cytokinesis. The microcarriers were simulated with different coating properties and the predicted mechanical stress levels were compared. The simulations show that both compressive and tensile stresses are present and that the stress distribution is strongly influenced by the microcarrier coating properties. These results give an indication that in the future, individual cell-based modeling methods may become a more widely used tool in combination with in vitro experiments in order to optimize cell culture processes.
Publication status: published
KU Leuven publication type: IC
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
Mechanical Engineering - miscellaneous
Biomechanics Section

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