Prebiotic effects of arabinoxylan-oligosaccharides (AXOS) on juvenile Siberian sturgeon (Acipenser baerii) with emphasis on the modulation of the gut microbiota using 454 pyrosequening

Geraylou, Zahra; Souffreau, Caroline; Rurangwa, Eugene; Maes, Gregory; Spanier, Katina; Courtin, Christophe; Delcour, Jan; Buyse, Johan; Ollevier, Frans

doi:10.1111/1574-6941.12169

Prebiotic effects of arabinoxylan-oligosaccharides (AXOS) on juvenile Siberian sturgeon (Acipenser baerii) with emphasis on the modulation of the gut microbiota using 454 pyrosequening

Author:

Geraylou, Zahra

Souffreau, Caroline ; Rurangwa, Eugene ; Maes, Gregory ; Spanier, Katina ; Courtin, Christophe ; Delcour, Jan ; Buyse, Johan ; Ollevier, Frans

Keywords:

Science & Technology, Life Sciences & Biomedicine, Microbiology, nondigestible carbohydrate, fish, gut microbial community, SCFA, IN-VITRO FERMENTATION, BACTERIAL FERMENTATION, GROWTH-PERFORMANCE, DIETARY INCLUSION, STOCKING DENSITY, AMUR STURGEON, INULIN, ARABINOXYLOOLIGOSACCHARIDES, FIBER, IMMUNOSTIMULANTS, Animals, Bacteria, Fatty Acids, Volatile, Fishes, Gastrointestinal Tract, Microbiota, Oligosaccharides, Prebiotics, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Xylans, 05 Environmental Sciences, 06 Biological Sciences, 11 Medical and Health Sciences, 3107 Microbiology

Abstract:

The potential of a novel class of prebiotics, arabinoxylan oligosaccharides (AXOS), was investigated on growth performance and gut microbiota of juvenile Acipenser baerii. Two independent feeding trials of 10 or 12 weeks were performed with basal diets supplemented with 2% or 4% AXOS-32-0.30 (trial 1) and 2% AXOS-32-0.30 or AXOS-3-0.25 (trial 2), respectively. Growth performance was improved by feeding 2% AXOS-32-0.30 in both trials, although not significantly. Microbial community profiles were determined using 454-pyrosequencing with barcoded primers targeting the V3 region of the 16S rRNA gene. AXOS significantly affected the relative abundance of bacteria at the phylum, family, genus and species level. The consumption of 2% AXOS-32-0.30 increased the relative abundance of Eubacteriaceae, Clostridiaceae, Streptococcaceae and Lactobacillaceae, while the abundance of Bacillaceae was greater in response to 4% AXOS-32-0.30 and 2% AXOS-3-0.25. The abundance of Lactobacillus spp. and Lactococcus lactis was greater after 2% AXOS-32-0.30 intake. Redundancy analysis showed a distinct and significant clustering of the gut microbiota of individuals consuming an AXOS diet. In both trials, concentration of acetate, butyrate and total short-chain fatty acids (SCFAs) increased in fish fed 2% AXOS-32-0.30. Our data demonstrate a shift in the hindgut microbiome of fish consuming different preparation of AXOS, with potential application as prebiotics.