Title: Cyclohexene nucleic acids (CeNA): Serum stable oligonucleotides that activate RNase H and increase duplex stability with complementary RNA
Authors: Wang, Jing ×
Verbeure, B
Luyten, Ingrid
Lescrinier, Eveline
Froeyen, Mathy
Hendrix, Christel
Rosemeyer, Helmut
Seela, Frank
Van Aerschot, Arthur
Herdewijn, Piet #
Issue Date: 2000
Publisher: Amer chemical soc
Series Title: Journal of the American Chemical Society vol:122 issue:36 pages:8595-8602
Abstract: The replacement of the furanose moiety of DNA by a cyclohexene ring gives a new nucleic acid structure: cyclohexene nucleic acids or CeNA. CeNAs can be obtained by the classical phosphoramidite chemisty starting from protected cyclohexenyl nucleoside building blocks. Incorporation of cylcohexenyl nucleosides in a DNA chain increases the stability of a DNA/RNA hybrid. The complex formed between cyclohexenyl oligoadenylate and its DNA or RNA complement is of similar stability. Circular dichroism (CD) and NMR studies indicate easy conformational adaptation of a cyclohexenyl nucleoside when incorporated in a natural nucleic acid structure. CeNA is stable against degradation in serum and a CeNA/RNA hybrid is able to activate E. Coli RNase H, resulting in cleavage of the RNA strand.
ISSN: 0002-7863
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Production Engineering, Machine Design and Automation (PMA) Section
Hypertension and Cardiovascular Epidemiology
Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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