Title: Anti-EGFR activation, anti-proliferative and pro-apoptotic effects of polyclonal antibodies induced by EGFR-based cancer vaccine
Authors: Ramírez, Belinda Sánchez *
Aguiar Alpizar, Yeranddy *
Fernández, Diana Rosa Hernández
Hidalgo, Greta Garrido
Capote, Ailem Rabasa
Rodríguez, Rolando Pérez
Fernández, Luis Enrique # ×
Issue Date: Sep-2008
Publisher: Butterworths
Series Title: Vaccine vol:26 issue:38 pages:4918-26
Article number: 10.1016/j.vaccine.2008.07.018
Abstract: Up to now clinical experiences focusing EGF receptor, an attractive target for cancer therapy, have been limited to passive therapies, suggesting that therapeutic cancer vaccines inducing anti-epidermal growth factor receptor (EGFR) antibodies could also work. Here, the humoral immune response induced in mice with a vaccine formulation containing the human EGFR-extracellular domain and very small-sized proteoliposomes (VSSP), a novel nanoparticulated adjuvant was assessed. In vaccinated mice sera average of the specific polyclonal antibodies (PAb) titers was 10(-5). Anti-EGFR PAb were able to bind EGFR+ tumor cell lines, expressing different levels of the molecule. Noteworthy, the presence of Cetuximab only partially inhibited the vaccine-induced antibodies binding to H125 cells. Anti-EGFR PAb abrogated ligands-dependent EGFR phosphorylation, provoking tumor cells apoptosis. The described EGFR-based vaccine might be a superior therapeutic approach for patients with EGFR+ tumors.
ISSN: 0264-410X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
* (joint) first author
× corresponding author
# (joint) last author

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