Title: HER1-ECD vaccination dispenses with emulsification to elicit HER1-specific anti-proliferative effects
Authors: Aguiar Alpizar, Yeranddy
Ramírez, Belinda Sánchez
Fernández, Diana Rosa Hernández
Capote, Ailem Rabasa
Hidalgo, Greta Garrido
Rodríguez, Rolando Pérez
Molina, Luis Enrique Fernández # ×
Issue Date: Mar-2009
Publisher: Landes Bioscience
Series Title: Human Vaccines vol:5 issue:3 pages:158-65
Abstract: EGFR (HER1) highlights as one of the most relevant tumor associated antigen in epithelial malignant cells. Monoclonal antibodies and tyrosine kinase inhibitors against EGFR remain as the most advanced approaches in clinical trials. More recently, an active immunotherapy using the HER1 extracellular domain (ECD) adjuvated in very small size proteoliposomes (VSSP) and emulsified in Montanide ISA-51 demonstrated its strength to inhibit tumor cell line proliferation by arresting cells in G(0)/G(1) stage and induction of apoptosis. In this study, we present a simpler HER1-ECD-based formulation, which is lacking the oily component Montanide ISA-51. Generated antibodies following non-emulsive formulation immunization recognized membrane EGFR; avoid EGF and TGFalpha coupling to EGFR leading to a marked abrogation of EGFR phosphorylation levels. Non-emulsive formulation also arrests cell cycle in G(0)/G(1) stage, demonstrating it preserves previous formulation quality in a newer and simpler formulation.
ISSN: 1554-8600
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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