Title: Hypericin-induced photosensitization of HeLa cells leads to apoptosis or necrosis - Involvement of cytochrome c and procaspase-3 activation in the mechanism of apoptosis
Authors: Vantieghem, Annelies
Assefa, Z
Vanden Abeele, P
Declercq, W
Courtois, S
Vandenheede, JR
Merlevede, Wilfried
de Witte, Peter
Agostinis, Patrizia #
Issue Date: Nov-1998
Publisher: Elsevier science bv
Series Title: FEBS Letters vol:440 issue:1-2 pages:19-24
Abstract: Here we report that photoactivated hypericin can induce either apoptosis or necrosis in HeLa cells, Under apoptotic conditions the cleavage of poly(ADP-ribose) polymerase (PARP) into the 85-kDa product is blocked by the caspase inhibitors benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (z-VAD-fmk) and benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone (z-DEVD-fmk). Both inhibitors protect cells from apoptosis but cannot prevent hypericin-induced necrosis, Conversely, HeLa cells overexpressing the viral cytokine response modifier A (CrmA), which inhibits caspase-1 and -8, still undergo hypericin-induced apoptosis and necrosis, Evidence is provided for the release of mitochondrial cytochrome c in the cytosol and for procaspase-3 activation in the hypericin-induced cell killing, (C) 1998 Federation of European Biochemical Societies.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Pharmaceutical Biology (-)
Biochemistry Section (Medicine) (-)
Laboratory of Cell Death Research & Therapy
# (joint) last author

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