Title: Development and validation of a liquid chromatography method for analysis of colistin sulphate
Authors: Orwa, JA ×
Van Gerven, A
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Apr-2000
Publisher: Vieweg
Series Title: Chromatographia vol:51 issue:7-8 pages:433-436
Abstract: The peptide antibiotic Colistin has been successfully separated into more than thirty components on a YMC-Pack Pro, C-18 column with a mobile phase of acetonitrile - sodium sulphate (0.7% m/v)- phosphoric acid (6.8% v/v dilution of 85% m/m H3PO4) - water (21.5:50:5:23.5) at 1.0 mt per min and detection at 215 nm. Baseline resolution of the major components, colistin A and B, and many minor components was obtained. Robustness was evaluated by per forming a full-factorial design experiment. The method showed good selectivity, repeatability, linearity and sensitivity.
ISSN: 0009-5893
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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