Title: Trafficking of Plant Vacuolar Invertases: From a Membrane-anchored to a Soluble Status. Understanding Sorting Information in Their Complex N-terminal Motifs
Authors: Xiang, Li
Van den Ende, Wim # ×
Issue Date: Sep-2013
Publisher: Japanese Society of Plant Physiologists
Series Title: Plant & Cell Physiology vol:54 issue:8 pages:1263-1277
Abstract: Vacuolar invertases (VIs) are highly expressed in young tissues and organs. They may have a
substantial regulatory influence on whole plant metabolism as well as on photosynthetic
efficiency. Therefore, they are emerging as potentially interesting biotechnological targets to
increase plant biomass production, especially under stress. On the one hand, VIs are well-known
as soluble and extractable proteins. On the other hand, they contain complex N-terminal
propeptide (NTPP) regions with a basic region (BR) and a transmembrane domain (TMD). Here
we analysed in depth the
Arabidopsis thaliana
VI2 (AtVI2) NTPP by mutagenesis. It was found
that correct sorting to the lytic vacuole (LV) depends on the presence of intact dileucine
(SSDALLPIS), BR (RRRR) and TMD motifs. AtVI2 remains inserted into membranes on its
way to the LV, and the classical sorting pathway (Endoplasmic Reticulum
LV) is
followed. However, our data suggest that VIs might follow an alternative, adaptor protein 3
(AP3)-dependent route as well. Membrane-anchored transport and a direct recognition of the
dileucine motif in the NTPP of VIs might have evolved as a simple and more efficient sorting
mechanism as compared to the vacuolar sorting receptor 1/binding protein of 80 kDa
(VSR1/BP80) dependent sorting mechanism followed by those proteins that travel to the vacuole
as soluble proteins.
ISSN: 0032-0781
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
× corresponding author
# (joint) last author

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