Title: Analysis of RNAi strategies against migratory parasitic nematodes of banana
Other Titles: Analyse van RNAi-strategieen tegen migratorische plantenparasitaire nematoden van banaan
Authors: Joseph, Soumi; R0226301
Issue Date: 18-Jun-2013
Abstract: The main objective of the research project is to studythe potentialof inducing an effective host resistance againstmigratory endo-parasitic nematodes through RNA- mediated interferencetechnology (RNAi).The study is mainly targeted at the migratoryendo-parasitic nematodes Radopholus similis and Pratylenchus coffeae ,which are the most important root pathogens of banana in the tropics.Since these nematodes invade the roots and move inter- andintra-cellularly through the root by disintegrating the cell wall asthey move along, the invasion and subsequent establishment of thenematode inthe plant can be prevented by knocking down nematode genesthat encode cell-wall degrading enzymes (e.g ?-1, 4-endoglucanase). Inaddition to genes specific to plant-parasitic nematodes such as ?-1,4-endoglucanase, genes that affect the parasitic life of the nematodeeitherdirectly or indirectly are also being targeted for silencing. Bydownregulating genes that play important roles in the embryonic andlarval development or nematode locomotion, the complete progression ofpathogenesis can be adversely affected. Based on this hypothesis, thisstudy aims to develop an alternative control strategy against migratoryendo-parasitic nematodes by knocking down the above mentionedparasitism or housekeeping genes. To achieve these goals, dsRNA will bedelivered to nematodes through in vitro and in planta routes. Thenematodes will be subjected to in vitro RNAi by soaking with dsRNA ofselected parasitism genes of the nematodes and these treated nematodesare then used to infect a host plant to investigate specific genes thataffect nematode parasitism. As a control strategy against the migratoryendo-parasitic nematodes, we attempt to engineer host plants, mainlybanana and chick pea to produce dsRNA of the nematode¬ís essential genesbased on the hypothesis that the in planta-produced dsRNA can triggerRNAi response in nematodes that feed on these transgenic plants.
Publication status: published
KU Leuven publication type: TH
Appears in Collections:Division of Crop Biotechnics

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