Title: Reversed phase high performance liquid chromatographic determination of cefixime in bulk drugs
Authors: Gonzalez-Hernandez, R ×
Nuevas-Paz, L
Soto-Mulet, L
Lopez-Lopez, M
Hoogmartens, Jos #
Issue Date: 2001
Publisher: Marcel dekker inc
Series Title: Journal of liquid chromatography & related technologies vol:24 issue:15 pages:2315-2324
Abstract: A new technique for the quantitative determination of cefixime trihydrate in bulk drugs by high performance liquid chromatography was developed using simple reagents. Finally, chosen conditions of analysis were as follows: LiChrospher 100 RP - 18 (250 x 4 mm I.D.) column, mobile phase consisting of phosphate buffer pH 7.0 and acetonitrile (93:7, v/v), flow rate of 0,8 mL/min, loop of 20 muL and UV detection at 287 nm. The prospective validation of this technique showed that it is linear in a range of 0.1 to 0.6 mg/mL (r = 0.9997), sensitive (0.3 %), precise (within-a-day repeatability, RSD = 1.0 %, day-to-day repeatability RSD = 1.3 %), accurate and selective (cefixime can be determined in presence of its related compounds). The limits of detection and quantitation are 37 ng (0.3 %) and 128 ng (1.1 %), respectively, relative to a 0.6 mg/mL solution.
ISSN: 1082-6076
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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