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Title: The highly autoaggregative and adhesive phenotype of the vaginal lactobacillus plantarum strain CMPG5300 Is sortase dependent
Authors: Malik, Shweta
Petrova, Mariya
Claes, Ingmar
Verhoeven, Tine
Busschaert, Pieter
Vaneechoutte, Mario #
Lievens, Bart
Lambrichts, Ivo
Siezen, Roland J.
Balzarini, Jan
Vanderleyden, Jos
Lebeer, Sarah # ×
Issue Date: Aug-2013
Publisher: American Society for Microbiology (ASM)
Series Title: Applied and Environmental Microbiology vol:79 pages:4576-85
Abstract: Lactobacilli are important for the maintenance of a healthy ecosystem in the human vagina.
Various mechanisms are postulated but so far poorly substantiated by molecular studies such as mutant analysis. Bacterial auto-aggregation is an interesting bacterial phenomenon, which can promote adhesion to host cells and displacement of pathogens. In this study, we report on the identification of a human vaginal isolate, Lactobacillus plantarum strain CMPG5300, which shows a high auto-aggregative and adhesive capacity. To investigate the importance of sortase dependent proteins (SDPs) in these phenotypes, a gene deletion mutant was constructed for srtA, the gene encoding the housekeeping sortase that covalently anchors these SDPs to the cell surface. This mutant lost the capacity to auto-aggregate, showed a decrease in adhesion to vaginal epithelial cells and lost the biofilm forming capacity under the conditions tested. These results indicate that the housekeeping sortase SrtA of CMPG5300 is a key determinant of the peculiar surface properties of this vaginal Lactobacillus strain.
ISSN: 0099-2240
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Microbial and Molecular Systems - miscellaneous
Laboratory of Virology and Chemotherapy (Rega Institute)
Bioengineering Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
Technologiecluster Bioengineering Technologie
Technologiecluster ESAT Elektrotechnische Engineering
Electrical Engineering (ESAT) TC, Technology Campus De Nayer Sint-Katelijne-Waver
× corresponding author
# (joint) last author

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