Title: Transient downregulation of Sema3A mRNA in a rat model for temporal lobe epilepsy - A novel molecular event potentially contributing to mossy fiber sprouting
Authors: Holtmaat, AJGD ×
Gorter, JA
de Wit, Joris
Tolner, EA
Spijker, S
Giger, RJ
da Silva, FHL
Verhaagen, J #
Issue Date: Jul-2003
Publisher: Academic Press
Series Title: Experimental Neurology vol:182 issue:1 pages:142-150
Abstract: Abstract
Mossy fiber sprouting (MFS) in the hippocampal dentate gyrus is thought to play a critical role in the hyperexcitability of the
hippocampus in temporal lobe epilepsy patients. The composition of molecular signals that is needed to direct this sprouting response has
not yet been elucidated to a great extent. In the present study we investigated the expression profile of Sema3A mRNA and the axonal
growth-associated protein GAP-43 mRNA during the process of electrically induced epileptogenesis in rats. Sema3A is an axon guidance
molecule with repellent activity on dentate granule cell axons. It is produced by neurons in the entorhinal cortex, which synapse on the
dendrites of dentate granule cells. Upregulation of GAP-43 expression in granule cells has often been reported in conjunction with MFS.
After induction of status epilepticus, the expression of Sema3A mRNA was temporarily downregulated in the entorhinal cortex concomitantly
with an upregulation of GAP-43 mRNA in dentate granule cells. In the following days, robust MFS into the dentate molecular layer
was observed. When the induction of status epilepticus was incomplete the two responses appeared to dissociate, i.e., the downregulation
of Sema3A mRNA did not occur, while upregulation of GAP-43 mRNA in dentate granule cells was still displayed. However, in these rats
no significant MFS was observed. These findings indicate that Sema3A mRNA downregulation is temporarily correlated with MFS, while
GAP-43 upregulation per se is not, and suggest that a loss of Sema3A in the molecular layer of the dentate gyrus could facilitate MFS into
this area during epilepsy.
ISSN: 0014-4886
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Holtmaat AJ-Exp Neurol 2003.pdfmain articlr Published 383KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science