Title: Semaphorin 3A displays a punctate distribution on the surface of neuronal cells and interacts with proteoglycans in the extracellular matrix
Authors: de Wit, Joris
De Winter, F
Klooster, J
Verhaagen, J # ×
Issue Date: May-2005
Publisher: Academic Press
Series Title: Molecular and Cellular Neurosciences vol:29 issue:1 pages:40-55
Abstract: Secreted semaphorins are essential for neural development and
continue to be expressed in subpopulations of adult neurons, where
they subserve as yet unknown functions. We employed functional mycand
GFP-tagged Sema3A proteins to obtain insight in the localization
of Sema3A in neuronal cells. Sema3A localized to both axons and
dendrites of cortical neurons. GFP-Sema3A exhibited a characteristic
punctate distribution on the surface of Neuro-2a cells, localized to
migratory pathways of cultured cells, and co-localized with and
induced clustering of its receptor component neuropilin-1. Treatment
with excess glycosaminoglycans and chondroitinase ABC resulted in
the removal of cell surface Sema3A. Heparin enhanced Sema3A’s
binding to neuropilin-1-expressing cells and potentiated its growth
cone collapsing activity. Together, these results indicate that association
with proteoglycans in the extracellular matrix of neuronal cells plays
an important role in the localization of the chemorepulsive guidance
cue Sema3A, and that this interaction may enhance its biological
ISSN: 1044-7431
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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