Title: Vesicular trafficking of semaphorin 3A is activity-dependent and differs between axons and dendrites
Authors: de Wit, Joris
Toonen, Ruud F
Verhaagen, Joost
Verhage, Matthijs # ×
Issue Date: Aug-2006
Publisher: Munksgaard
Series Title: Traffic vol:7 issue:8 pages:1060-1077
Abstract: Secreted semaphorins act as guidance cues in the developing
nervous system and may have additional functions
in mature neurons. How semaphorins are transported and
secreted by neurons is poorly understood. We find that
endogenous semaphorin 3A (Sema3A) displays a punctate
distribution in axons and dendrites of cultured cortical
neurons. GFP–Sema3A shows a similar distribution and
co-localizes with secretory vesicle cargo proteins. Live-cell
imaging reveals highly dynamic trafficking of GFP–
Sema3A vesicles with distinct properties in axons and
dendrites regarding directionality, velocity, mobility and
pausing time. In axons, most GFP–Sema3A vesicles move
fast without interruption, almost exclusively in the anterograde
direction, while in dendrites many GFP–Sema3A
vesicles are stationary and move equally frequent in both
directions. Disruption of microtubules, but not of actin
filaments, significantly impairs GFP–Sema3A transport.
Interestingly, depolarization induces a reversible arrest of
axonal transport of GFP–Sema3A vesicles but has little
effect on dendritic transport. Conversely, action potential
blockade using tetrodotoxin (TTX) accelerates axonal
transport, but not dendritic transport. These data indicate
that axons and dendrites regulate trafficking of Sema3A
and probably other secretory vesicles in distinct ways,
with axons specializing in fast, uninterrupted, anterograde
transport. Furthermore, neuronal activity regulates secretory
vesicle trafficking in axons by a depolarizationevoked
trafficking arrest.
ISSN: 1398-9219
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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