Title: Influence of organic matter on flocculation of Chlorella vulgaris by calcium phosphate precipitation
Authors: Beuckels, Annelies * ×
Depraetere, Orily *
Vandamme, Dries
Foubert, Imogen
Smolders, Erik
Muylaert, Koenraad #
Issue Date: Jul-2013
Publisher: Pergamon
Series Title: Biomass & Bioenergy vol:54 pages:107-114
Abstract: Flocculation is a promising approach for reducing the cost of harvesting microalgae.
Flocculation of microalgae can be induced by precipitation of calcium phosphate (Ca-phosphate) when
pH increases above 8.5, a pH level that can be achieved by simple photosynthetic CO2 depletion. Using
the freshwater microalgae Chlorella vulgaris as a model, we identified the combinations of minimum
pH and Ca and PO4 concentrations to induce flocculation. Predicted concentrations of amorphous
Ca3(PO4)2 precipitation (chemical modelling) explained flocculation in these solutions. The efficiency
of flocculation decreased with increasing microalgal biomass concentration. Solution renewal
experiments suggest that flocculation is inhibited by algal organic matter in the medium, even when
present at relatively low concentrations relative to concentrations in stationary phase medium.
Addition of dissolved organic compounds showed that organic acids with a high molecular weight (e.g.
humic acids, alginate) have a strong inhibitory effect on flocculation whereas glucose or acetate had no
such effect. These effects may be related to complexation of Ca2+ or effects of organic matter on
growth of the Ca-phosphate crystals. Precipitation of Ca-phosphate in media with high organic matter
content requires a high water hardness (500 μmol L-1 Ca) and high PO4 concentrations (350 μmol L-1
P). Flocculation can be facilitated by addition of surplus PO4 to the medium. This surplus PO4 may be
recovered after flocculation by re-dissolution of the Ca-phosphate through mild acidification.
ISSN: 0961-9534
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biology, Campus Kulak Kortrijk
Microbial and Molecular Systems, Campus Kulak Kortrijk
Division Soil and Water Management
* (joint) first author
× corresponding author
# (joint) last author

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