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Title: Probabilistic Identification of Cerebellar Cortical Neurones across Species
Authors: Van Dijck, Gert ×
Van Hulle, M.M.
Heiney, Shane A
Blazquez, Pablo M
Meng, Hui
Angelaki, Dora E
Arenz, Alexander
Margrie, Troy W
Mostofi, Abteen
Edgley, Steve
Bengtsson, Fredrik
Ekerot, Carl-Fredrik
Jörntell, Henrik
Dalley, Jeffrey W
Holtzman, Tahl #
Issue Date: 4-Mar-2013
Publisher: Public Library of Sciene
Series Title: PLoS One vol:8 issue:3 pages:e57669
Article number: 10.1371/journal.pone.0057669
Abstract: Despite our fine-grain anatomical knowledge of the cerebellar cortex, electrophysiological studies of circuit information processing over the last fifty years have been hampered by the difficulty of reliably assigning signals to identified cell types. We approached this problem by assessing the spontaneous activity signatures of identified cerebellar cortical neurones. A range of statistics describing firing frequency and irregularity were then used, individually and in combination, to build Gaussian Process Classifiers (GPC) leading to a probabilistic classification of each neurone type and the computation of equi-probable decision boundaries between cell classes. Firing frequency statistics were useful for separating Purkinje cells from granular layer units, whilst firing irregularity measures proved most useful for distinguishing cells within granular layer cell classes. Considered as single statistics, we achieved classification accuracies of 72.5% and 92.7% for granular layer and molecular layer units respectively. Combining statistics to form twin-variate GPC models substantially improved classification accuracies with the combination of mean spike frequency and log-interval entropy offering classification accuracies of 92.7% and 99.2% for our molecular and granular layer models, respectively. A cross-species comparison was performed, using data drawn from anaesthetised mice and decerebrate cats, where our models offered 80% and 100% classification accuracy. We then used our models to assess non-identified data from awake monkeys and rabbits in order to highlight subsets of neurones with the greatest degree of similarity to identified cell classes. In this way, our GPC-based approach for tentatively identifying neurones from their spontaneous activity signatures, in the absence of an established ground-truth, nonetheless affords the experimenter a statistically robust means of grouping cells with properties matching known cell classes. Our approach therefore may have broad application to a variety of future cerebellar cortical investigations, particularly in awake animals where opportunities for definitive cell identification are limited.
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Research Group Neurophysiology
× corresponding author
# (joint) last author

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