ITEM METADATA RECORD
Title: Emergence of cowpox: study of the virulence of clinical strains and evaluation of antivirals
Authors: Duraffour, Sophie ×
Mertens, Barbara
Meyer, Hermann
van den Oord, Joost J.
Mitera, Tania
Matthys, Patrick
Snoeck, Robert
Andrei, Graciela #
Issue Date: Feb-2013
Publisher: Public Library of Sciene
Series Title: PLoS One vol:8 issue:2 pages:e55808
Article number: 10.1371/journal.pone.0055808
Abstract: The last years, cowpox infections are being increasingly reported through Eurasia. Cowpox viruses (CPXVs) have been reported to have different genotypes and may be subdivided in at least five genetically distinct monophyletic clusters. However, little is known about their and features. In this report, five genetically diverse CPXVs, including one reference strain (CPXV strain Brighton) and four clinical isolates from human and animal cases, were compared with regard to growth in cells, pathogenicity in mice and inhibition by antivirals. While all CPXVs replicated similarly and showed comparable antiviral susceptibility, marked discrepancies were seen , including differences in virulence with recorded mortality rates of 0%, 20% and 100%. The four CPXV clinical isolates appeared less pathogenic than two reference strains, CPXV Brighton and vaccinia virus Western-Reserve. Disease severity seemed to correlate with high viral DNA loads in several organs, virus titers in lung tissues and levels of IL-6 cytokine in the sera. Our study highlighted that the species CPXV consists of viruses that not only differ considerably in their genotypes but also in their phenotypes, indicating that CPXVs should not be longer classified as a single species. Lung virus titers and IL-6 cytokine level in mice may be used as biomarkers for predicting disease severity. We further demonstrated the potential benefit of cidofovir, CMX001 and ST-246 use as antiviral therapy.
URI: 
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Virology and Chemotherapy (Rega Institute)
Translational Cell & Tissue Research
Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
2013022.pdf Published 2171KbAdobe PDFView/Open

 


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science