Title: A Novel Depsipeptide Produced by Paenibacillus alvei 32 Isolated from a Cystic fibrosis Patient
Authors: Chevrot, Romain ×
Didelot, Sandrine
Van den Bossche, Larissa
Tambadou, Fatoumata
Caradec, Thibault
Marchand, Pierre
Izquierdo, Esther
Sopéna, Valérie
Caillon, Jocelyne
Barthélémy, Cyrille
Van Schepdael, Ann
Hoogmartens, Jos
Rosenfeld, Eric #
Issue Date: Jan-2013
Publisher: Springer New York LLC
Series Title: Probiotics and Antimicrobial Proteins vol:5 pages:18-25
Abstract: The important viscosity of the respiratory tract
mucus of Cystic fibrosis (CF) patients impairs the mucociliary transport system and allows the growth of numerous micro-organisms. Among them, Pseudomonas aeruginosa
and Staphylococcus aureus are known to be responsible for
pulmonary infections. We imagined that CF microflora
could also harbour micro-organisms naturally equipped to
compete with these pathogens. A method was developed to
recover these antibiotic-producing strains within 20 CF
sputum. Using this approach, we have isolated an unusual
Gram-positive bacterium identified as Paenibacillus alvei
by Api galleries and 16S rRNA gene sequence analysis.
This strain has inhibited the growth of P. aeruginosa,
S. aureus and Klebsiella pneumoniae, in co-cultures. A
liquid mineral medium named MODT50 was designed and
optimised for the production and the recovery of the antimicrobial compounds. The supernatant has inhibited the
growth of all Gram-positive strains tested, even Methicillin-resistant S. aureus. One antimicrobial compound with a peptide structure (mainly active against S. aureus, Micrococcus luteus, and Pseudomonas stutzeri) has been purified and characterised by liquid chromatography-mass
spectrometry. The new active molecule (m/z 786.6)
named depsipeptide L possesses a 15-guanidino-3-hydroxypentadecanoic acid side chain (m/z 298) linked on a
cyclic part of four amino acids residues (Ser, two Leu/Ile,
Arg). This work reports for the first time the production of
such a molecule by a P. alvei strain in a mineral medium.
The CF lung microflora might represent a valuable source
for the discovery of new antimicrobial-producing strains.
ISSN: 1867-1306
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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