Title: Zymography methods for visualizing hydrolytic enzymes
Authors: Vandooren, Jennifer ×
Geurts, Nathalie
Martens, Erik
Van den Steen, Philippe
Opdenakker, Ghislain #
Issue Date: Mar-2013
Publisher: Nature Pub. Group
Series Title: Nature Methods vol:10 issue:3 pages:211-220
Article number: 10.1038/nmeth.2371
Abstract: Zymography is a technique for studying hydrolytic enzymes on the basis of substrate degradation. It is a powerful, but often misinterpreted, tool yielding information on potential hydrolytic activities, enzyme forms and the locations of active enzymes. In this Review, zymography techniques are compared in terms of advantages, limitations and interpretations. With in gel zymography, enzyme forms are visualized according to their molecular weights. Proteolytic activities are localized in tissue sections with in situ zymography. In vivo zymography can pinpoint proteolytic activity to sites in an intact organism. Future development of novel substrate probes and improvement in detection and imaging methods will increase the applicability of zymography for (reverse) degradomics studies.
ISSN: 1548-7091
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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