Title: Assembly of the translocase motor onto the preprotein-conducting channel
Authors: Karamanou, Spyridoula ×
Bariami, Vassiliki
Papanikou, Efrosyni
Kalodimos, Charalampos G
Economou, Anastassios #
Issue Date: Oct-2008
Publisher: Blackwell Science
Series Title: Molecular Microbiology vol:70 issue:2 pages:311-22
Article number: 10.1111/j.1365-2958.2008.06402.x
Abstract: Bacterial protein secretion is catalysed by the SecYEG protein-conducting channel complexed with the SecA ATPase motor. To gain insight into the SecA-SecYEG interaction we used peptide arrays, thermodynamic quantification, mutagenesis and functional assays. Our data reveal that: (i) SecA binds with low affinity on several, peripheral, exposed SecYEG sites. This largely electrostatic association is modulated by temperature and nucleotides. (ii) Binding sites cluster in five major binding 'regions': three that are exclusively cytoplasmic and two that reach the periplasm. (iii) Both the N-terminal and c-terminal regions of SecA participate in binding interactions and share some sites. (iv) Several of these sites are essential for translocase catalysis. Our data provide residue-level dissection of the SecYEG-SecA interaction. Two models of assembly of SecA on dimeric SecYEG are discussed.
ISSN: 0950-382X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science