Title: Preprotein-controlled catalysis in the helicase motor of SecA
Authors: Karamanou, Spyridoula ×
Gouridis, Giorgos
Papanikou, Efrosyni
Sianidis, Giorgos
Gelis, Ioannis
Keramisanou, Dimitra
Vrontou, Eleftheria
Kalodimos, Charalampos G
Economou, Anastassios #
Issue Date: Jun-2007
Publisher: Nature Publishing Group
Series Title: EMBO Journal vol:26 issue:12 pages:2904-14
Abstract: The cornerstone of the functionality of almost all motor proteins is the regulation of their activity by binding interactions with their respective substrates. In most cases, the underlying mechanism of this regulation remains unknown. Here, we reveal a novel mechanism used by secretory preproteins to control the catalytic cycle of the helicase 'DEAD' motor of SecA, the preprotein translocase ATPase. The central feature of this mechanism is a highly conserved salt-bridge, Gate1, that controls the opening/closure of the nucleotide cleft. Gate1 regulates the propagation of binding signal generated at the Preprotein Binding Domain to the nucleotide cleft, thus allowing the physical coupling of preprotein binding and release to the ATPase cycle. This relay mechanism is at play only after SecA has been previously 'primed' by binding to SecYEG, the transmembrane protein-conducting channel. The Gate1-controlled relay mechanism is essential for protein translocase catalysis and may be common in helicase motors.
ISSN: 0261-4189
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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