Title: Double hexameric ring assembly of the type III protein translocase ATPase HrcN
Authors: Müller, Shirley A ×
Pozidis, Charalambos
Stone, Remington
Meesters, Christian
Chami, Mohamed
Engel, Andreas
Economou, Anastassios
Stahlberg, Henning #
Issue Date: Jul-2006
Publisher: Blackwell Science
Series Title: Molecular Microbiology vol:61 issue:1 pages:119-25
Abstract: The specialized type III secretion (T3S) apparatus of pathogenic and symbiotic Gram-negative bacteria comprises a complex transmembrane organelle and an ATPase homologous to the F1-ATPase beta subunit. The T3S ATPase HrcN of Pseudomonas syringae associates with the inner membrane, and its ATP hydrolytic activity is stimulated by dodecamerization. The structure of dodecameric HrcN (HrcN12) determined to 1.6 nm by cryo-electron microscopy is presented. HrcN12 comprises two hexameric rings that are probably stacked face-to-face by the association of their C-terminal domains. It is 11.5 +/- 1.0 nm in diameter, 12.0 +/- 2.0 nm high and has a 2.0-3.8 nm wide inner channel. This structure is compared to a homology model based on the structure of the F1-beta-ATPase. A model for its incorporation within the T3S apparatus is presented.
ISSN: 0950-382X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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