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Title: Cloning, purification and characterization of a functional anthracycline glycosyltransferase
Authors: Sianidis, Giorgos ×
Wohlert, Sven-Eric
Pozidis, Charalambos
Karamanou, Spyridoula
Luzhetskyy, Andriy
Vente, Andreas
Economou, Anastassios #
Issue Date: Sep-2006
Publisher: Elsevier BV
Series Title: Journal of Biotechnology vol:125 issue:3 pages:425-33
Abstract: We have cloned the gene that encodes a novel glucosyl transferase (AraGT) involved in rhamnosylation of the polyketide antibiotic Aranciamycin in Streptomyces echinatus. AraGT comprises two domains characteristic of bacterial glycosyltranferases. AraGT was synthesized in E. coli as a decahistidinyl-tagged polypeptide. Purified AraGT is dimeric, displays a T(mapp) of 30 degrees C and can glycosylate the aglycone of an Aranciamycin derivative as shown by liquid chromatography and mass spectrometry. The availability of functional AraGT will allow the generation Aranciamycin-based combinatorial libraries.
URI: 
ISSN: 0168-1656
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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