Title: Rhophilin-2 is targeted to late-endosomal structures of the vesicular machinery in the presence of activated RhoB
Authors: Steuve, Séverine
Devosse, Thalie
Lauwers, Elsa
Vanderwinden, Jean-Marie
André, Bruno ×
Courtoy, Pierre J
Pirson, Isabelle #
Issue Date: Dec-2006
Publisher: Academic Press
Series Title: Experimental Cell Research vol:312 issue:20 pages:3981-9
Abstract: Rhophilin-2 or p76(RBE), a protein whose expression is induced by the cyclic AMP pathway in thyrocytes, contains several protein-protein interaction domains including HR-1, Bro1 and PDZ domains, and is a partner of RhoB in its GTP-bound form (Eur J Biochem, 269(24): 6241-9, 2002). We here define its subcellular localization and dissect the significance of its domains. By subcellular fractionation and colocalization experiments, rhophilin-2 is recruited to subcellular organelles by activated RhoB-GTP. As for its yeast homologue, Npi3/Bro1p, the Bro1 domain of rhophilin-2 is necessary to its recruitment to the vesicular structures, which are not labeled for EEA1 nor Lamp1, but well with the late endosome marker CD63.
ISSN: 0014-4827
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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