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Title: Substituting Coomassie Brilliant Blue for bromophenol blue in two-dimensional electrophoresis buffers improves the resolution of focusing patterns
Authors: Vilain, S
Cosette, P
Charlionet, R
Hubert, M
Lange, C
Junter, GA
Jouenne, T #
Issue Date: 2001
Publisher: Wiley-v c h verlag gmbh
Series Title: Electrophoresis vol:22 issue:20 pages:4368-4374
Abstract: In a new area of postgenomics challenges, the optimization of protein identification has become a central goal in microbiochemistry. In this work, we demonstrate that the substitution of Coomassie Brilliant Blue for bromophenol blue in two-dimensional electrophoresis (2-DE) buffers improves the focusing of whole proteins from Pseudomonas aeruginosa. This improvement of focusing concerns more particularly basic proteins. This enhancement may be attributed to a better transfer from the first to the second dimension, which probably highlights an increase in the solubility of proteins in the IPG strips. Hence, the use of an efficient tracking dye in the 2-DE buffers may enlarge protein recovery on proteome maps.
URI: 
ISSN: 0173-0835
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Neuronal Communication
# (joint) last author

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