Title: Affinity chromatography with immobilised endoxylanases separates TAXI- and XIP-type endoxylanase inhibitors from wheat (Triticum aestivum L.)
Authors: Gebruers, Kurt ×
Brijs, Kristof
Courtin, Christophe
Goesaert, Hans
Proost, Paul
Van Damme, Jozef
Delcour, Jan #
Issue Date: 2002
Publisher: Academic press ltd elsevier science ltd
Series Title: Journal of cereal science vol:36 issue:3 pages:367-75
Abstract: An affinity-based purification procedure allowed the resolution of two distinct groups of endoxylanase inhibitors with different molecular structures and endoxylanase specificities from wheat wholemeal. The first group comprises the so-called Triticum aestivum L. Endoxylanase inhibitor (TAXI)-type proteins which are of approx. M-r 40 000 and occur in two different molecular forms. These inhibitors were removed from a concentrated cation exchange chromatography fraction from wheat wholemeal on a Bacillus subtilis endoxylanase affinity column. The second group of structurally different endoxylanase inhibitors, the so-called xylanase inhibiting protein (XIP)-type, of approx. M-r 29 000-32 000, with pI values varying between 8.8 and 9.2, was purified from the unbound fraction from the B. subtilis endoxylanase affinity column by chromatography on an Aspergillus niger endoxylanase affinity column followed by gel permeation chromatography. The XIP-type inhibitors are not active against the B. subtilis endoxylanase and were consequently not retained on the B. subtilis endoxylanase column. Further analysis of the XIP-type proteins by high-resolution cation exchange chromatography, SDS-PAGE and iso-electrofocusing, revealed several forms. They had similar endoxylanase specificities and N-terminal amino acid sequences. (C) 2002 Published by Elsevier Science Ltd.
ISSN: 0733-5210
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Laboratory of Molecular Immunology (Rega Institute)
Academic Center for General Practice
× corresponding author
# (joint) last author

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