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Title: Simultaneous isolation of wheat high molecular weight and low molecular weight glutenin subunits
Authors: Verbuggen, IM ×
Veraverbeke, WS
Vandamme, A
Delcour, Jan #
Issue Date: 1998
Publisher: Academic press ltd
Series Title: Journal of cereal science vol:28 issue:1 pages:25-32
Abstract: A procedure was established for isolating simultaneously high molecular weight (HMW-GS) and low molecular weight glutenin subunits (LMW-GS) from chloroform-defatted wheat flour. Temperature conditions were optimised to obtain maximum yield and purity. A pre-extraction of gliadin was performed with 50% (v/v) propan-1-ol at room temperature. Glutenin subunits were solubilised from the residue at 60 degrees C with 50% (v/v) propan-1-ol containing 1% (w/v) dithiothreitol. The HMW-GS in the glutenin mixture were then precipitated selectively by increasing the propan-1-ol concentration to 60%. Upon addition of propan-1-ol to a final concentration of 85%, LMW-GS were precipitated. It was shown by densitometric scanning of SDS-PAGE gels that the isolated glutenin subunits were very pure. Furthermore, a partial separation of LMW-GS into B and C type subunits was observed when the propan-1-ol concentration was increased gradually. (C) 1998 Academic Press.
ISSN: 0733-5210
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Academic Center for General Practice
Teaching Support Unit, Faculty of Bioscience Engineering
× corresponding author
# (joint) last author

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