Title: Dormant phase and multinuclear cells: two key phenomena in early culture of murine bone marrow mesenchymal stem cells
Authors: Ahmadbeigi, Naser ×
Soleimani, Masoud
Gheisari, Yousof
Vasei, Mohammad
Amanpour, Saeid
Bagherizadeh, Iman
Shariati, Seyed Ali Mohammad
Azadmanesh, Kayhan
Amini, Sepideh
Shafiee, Abbas
Arabkari, Vahid
Nardi, Nance Beyer #
Issue Date: Aug-2011
Publisher: Mary Ann Liebert, Inc.
Series Title: Stem Cells and Development vol:20 issue:8 pages:1337-47
Article number: 10.1089/scd.2010.0266
Abstract: Special features of mesenchymal stem cells (MSCs) have made them a popular tool in cell therapy and tissue engineering. Although mouse animal models and murine MSCs are common tools in this field, our understanding of the effect of in vitro expansion on the behavior of these cells is poor and controversial. In addition, in comparison to human, isolation of MSCs from mouse has been reported to be more difficult and some unexplained features such as heterogeneity and slow growth rate in the culture of these cells have been observed. Here we followed mouse bone marrow MSCs for >1 year after isolation and examined the effect of expansion on changes in morphology, growth kinetics, plasticity, and chromosomal structure during in vitro culture. Shortly after isolation, the growth rate of the cells decreased until they stopped dividing and entered a dormant state. In this state the size of the cells increased and they became multinuclear. These large multinuclear cells then gave origin to small mononuclear cells, which after a while resumed proliferation and could be expanded immortally. The immortal cells had diminished plasticity and were aneuploid but could not form tumors in nude mice. These results suggest that mouse bone marrow MSCs bear several modifications when expanded in vitro, and therefore, the interpretation of the data obtained with these cells should be done more cautiously.
ISSN: 1547-3287
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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