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Title: Riboswitch conformations revealed by small-angle X-ray scattering
Authors: Lipfert, Jan
Herschlag, Daniel
Doniach, Sebastian # ×
Issue Date: 2009
Series Title: Methods in Molecular Biology vol:540 pages:141-59
Article number: 10.1007/978-1-59745-558-9_11
Abstract: Riboswitches are functional RNA molecules that control gene expression through conformational changes in response to small-molecule ligand binding. In addition, riboswitch 3D structure, like that of other RNA molecules, is dependent on cation-RNA interactions as the RNA backbone is highly negatively charged. Here, we show how small-angle X-ray scattering (SAXS) can be used to probe RNA conformations as a function of ligand and ion concentration. In a recent study of a glycine-binding tandem aptamer from Vibrio cholerae, we have used SAXS data and thermodynamic modeling to investigate how Mg(2+)-dependent folding and glycine binding are energetically coupled. In addition, we have employed ab initio shape reconstruction algorithms to obtain low-resolution models of the riboswitch structure from SAXS data under different solution conditions.
URI: 
ISSN: 1064-3745
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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