Title: Directed migration of cortical interneurons depends on the cell-autonomous action of sip1
Authors: van den Berghe, Veronique ×
Stappers, Elke
Vandesande, Bram
Dimidschstein, Jordane
Kroes, Roel
Francis, Annick
Conidi, Andrea
Lesage, Flore
Dries, Ruben
Cazzola, Silvia
Berx, Geert
Kessaris, Nicoletta
Vanderhaeghen, Pierre
van Ijcken, Wilfred
Grosveld, Frank G
Goossens, Steven
Haigh, Jody J
Fishell, Gord
Goffinet, André
Aerts, Stein
Huylebroeck, Danny
Seuntjens, Eve #
Issue Date: Jan-2013
Publisher: Cell Press
Series Title: Neuron vol:77 issue:1 pages:70-82
Article number: 10.1016/j.neuron.2012.11.009
Abstract: GABAergic interneurons mainly originate in the medial ganglionic eminence (MGE) of the embryonic ventral telencephalon (VT) and migrate tangentially to the cortex, guided by membrane-bound and secreted factors. We found that Sip1 (Zfhx1b, Zeb2), a transcription factor enriched in migrating cortical interneurons, is required for their proper differentiation and correct guidance. The majority of Sip1 knockout interneurons fail to migrate to the neocortex and stall in the VT. RNA sequencing reveals that Sip1 knockout interneurons do not acquire a fully mature cortical interneuron identity and contain increased levels of the repulsive receptor Unc5b. Focal electroporation of Unc5b-encoding vectors in the MGE of wild-type brain slices disturbs migration to the neocortex, whereas reducing Unc5b levels in Sip1 knockout slices and brains rescues the migration defect. Our results reveal that Sip1, through tuning of Unc5b levels, is essential for cortical interneuron guidance.
ISSN: 0896-6273
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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