Title: BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl
Authors: Hemmeryckx, Bianca ×
Reichert, A
Watanabe, M
Kaartinen, V
de Jong, R
Pattengale, PK
Groffen, J
Heisterkamp, N #
Issue Date: 2002
Publisher: Scientific & Medical Division, Macmillan Press
Series Title: Oncogene vol:21 issue:20 pages:3225-3231
Abstract: The Bcr/Abl fusion protein directly causes chronic myelogenous leukemia and Philadelphia-chromosome positive acute lymphoblastic leukemia. Multiple independent studies have implicated Crkl, a small adapter protein, in transduction of oncogenic signals of Bcr/Abl and Crkl tyrosine-phosphorylation is used as a diagnostic tool for Philadelphia-positive leukemia. To evaluate the contribution of Crkl to this type of leukemia, we generated mutant mice that lack Crkl expression. We found that the overall survival of P190 BCR/ABL crkl-/- mice was comparable to that of genetically matched P190 BCR/ABL crkl +/+ mice. Both genotypes developed lymphoid lineage leukemia/lymphoma. Western blot analysis of -/- and +/+ lymphomas showed that the related Crk protein was tyrosine phosphorylated and could be found complexed with Bcr-Abl P190. These data indicate that possible therapeutic approaches that target Crkl may be complicated by the presence of pathways that compensate for lack of Crkl function.
ISSN: 0950-9232
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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