Title: Trypsin-mediated semisynthesis of salmon-calcitonin
Authors: Davey, Mark
Rommelaere, H
Deboeck, S
Goethals, M
Vandamme, J
Van de Kerckhove, J #
Issue Date: 1995
Publisher: Munksgaard int publ ltd
Series Title: International journal of peptide and protein research vol:45 issue:4 pages:380-385
Abstract: Salmon calcitonin, CT(1-32). NH2, was synthesised by the trypsin-mediated coupling of the peptide fragments CT(1-24) and CT(25-32). NH2, prepared by conventional Fmoc solid-phase chemistry. Optimal conditions regarding reaction time course, pH, proportion of catalyst, substrate concentration and composition of the reaction medium were determined from initial studies on the coupling of CT(1-11) to CT(12-24) and of CT(12-24) to CT(25-32). NH2. For the final successful semisynthesis, we found that it was unnecessary to protect lysine residues not involved in the coupling, and that secondary hydrolysis at these sites could be prevented by increasing the pH of the reaction medium. The reaction achieved equilibrium after 30-45 min, with overall conversion of around 30% of the initial amount of CT(1-24) substrate into product. Yields were depressed due to cyclisation of the CT(1-24) substrate via air-oxidation of the Cys(1) and Cys(7) residues. (C) Munksgaard 1995.
ISSN: 0367-8377
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
# (joint) last author

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