Title: Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy
Authors: Dedecker, Peter ×
Duwé, Sam
Neely, Robert
Zhang, Jin #
Issue Date: 3-Dec-2012
Publisher: Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
Series Title: Journal of Biomedical Optics vol:17 issue:12 pages:1-5
Abstract: We present Localizer, a freely available and open source software package that implements the com- putational data processing inherent to several types of superresolution fluorescence imaging, such as localization (PALM/STORM/GSDIM) and fluctuation imaging (SOFI/pcSOFI). Localizer delivers high accuracy and performance and comes with a fully featured and easy-to-use graphical user interface but is also designed to be integrated in higher-level analysis environments. Due to its modular design, Localizer can be readily extended with new algo- rithms as they become available, while maintaining the same interface and performance. Weprovide front-ends for running Localizer from Igor Pro, Matlab, or as a stand-alone program. We show that Localizer performs favorably when compared with two existing superresolution packages, and to our knowledge is the only freely available implementation of SOFI/pcSOFI microscopy. By dramatically improving the analysis performance and ensuring the easy addition of current and future enhancements, Localizer strongly improves the usability of superresolution imaging in a variety of biomedical studies.
ISSN: 1083-3668
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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