Title: Nucleic acids for ultra-sensitive protein detection
Authors: Janssen, Kris
Knez, Karel
Spasic, Dragana
Lammertyn, Jeroen # ×
Issue Date: 14-Jan-2013
Publisher: Molecular Diversity Preservation International (MDPI)
Series Title: Sensors vol:13 pages:898-930
Article number: 26425
Abstract: Major advancements in molecular biology and clinical diagnostics cannot be brought about strictly through the use of genomics based methods. Improved methods for protein detection and proteomic screening are an absolute necessity to complement to wealth of information offered by novel, high-throughtput sequencing technologies. Only then will it be possible to advance insights into clinical processes and to characterize the importance of specific protein biomarkers for disease detection or the realization of `personalized medicine'. Currently however, large-scale proteomic information is still not as easily obtained as its genomic counterpart mainly because traditional, antibody-based technologies struggle to meet the stringent sensitivity- and throughput requirements that are required whereas mass-spectrometry based methods might be burdened by significant costs involved. However, recent years have seen the development of new biodetection strategies linking nucleic acids with existing antibody technology or replacing antibodies with oligonucleotide recognition elements altogether. These advancements have unlocked many new strategies to lower detection limits and dramatically increase throughput of protein detection assays. In this review, an overview of these new strategies will be given.
ISSN: 1424-8220
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
× corresponding author
# (joint) last author

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