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Title: Isolation of cereal arabinogalactan-peptides and structural comparison of their carbohydrate and peptide moieties
Authors: Van den Bulck, K ×
Swennen, Katrien
Loosveld, A-M A
Courtin, Christophe
Brijs, Kristof
Proost, Paul
Van Damme, Jozef
Van Campenhout, Steven
Mort, A
Delcour, Jan #
Issue Date: Jan-2005
Publisher: Academic Press
Series Title: Journal of Cereal Science vol:41 issue:1 pages:59-67
Abstract: Arabinogalactan (AG)-peptides were isolated and purified from wheat and durum wheat and for the first time from spelt, triticale, rye and barley using three dedicated isolation procedures. The AG-peptide molecules have molecular weights of approximately 23,500 with the exception of triticale (27,500) and rye (33,000). The fine structure of the carbohydrate parts revealed close resemblances among the purified cereal AG-peptide samples. They consist of a (1 --> 6)-beta-D-galactopyranosyl backbone substituted in the C(O)3-position with a single alpha-L-arabinofuranosyl or a single beta-D-galactopyranosyl residue. The latter can also be substituted in its C(O)3-position with a single alpha-L-arabinofuranosyl residue. The AG-peptide peptide cores typically exist of 15 amino acids including three highly conserved hydroxyprolines (Hyp), each linked to a carbohydrate chain. The peptide amino acid sequence of spelt and durum wheat AG-peptides showed high similarity with the wheat AG-peptide peptide sequence while triticale, rye and barley AG-peptide peptide cores displayed less similarity. Homology with the N-terminal part of cereal grain softness protein (GSP) precursors indicates that the cereal AG-peptide peptides are a processing product of GSP synthesis. An overall structural model is proposed. (C) 2004 Elsevier Ltd. All rights reserved.
URI: 
ISSN: 0733-5210
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Laboratory of Molecular Immunology (Rega Institute)
× corresponding author
# (joint) last author

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