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Title: A PKS/NRPS/FAS hybrid gene cluster from Serratia plymuthica RVH1 encoding the biosynthesis of three broad spectrum, Zeamine-related antibiotics
Authors: Masschelein, Joleen
Mattheus, Wesley
Gao, Ling-Jie
Moons, Pieter
Van Houdt, Rob
Uytterhoeven, Birgit
Lamberigts, Chris
Lescrinier, Eveline
Rozenski, Jef
Herdewijn, Piet
Aertsen, Abram
Michiels, Chris
Lavigne, Rob # ×
Issue Date: 2013
Publisher: Public Library of Sciene
Series Title: PLoS One vol:8 issue:1
Article number: e54143
Abstract: Serratia plymuthica strain RVH1, initially isolated from an industrial food processing environment, displays potent antimicrobial activity towards a broad spectrum of Gram-positive and Gram-negative bacterial pathogens. Isolation and subsequent structure determination of bioactive molecules led to the identification of two polyamino antibiotics with the same molecular structure as zeamine and zeamine II as well as a third, closely related analogue, designated zeamine I. The gene cluster encoding the biosynthesis of the zeamine antibiotics was cloned and sequenced and shown to encode FAS, PKS as well as NRPS related enzymes in addition to putative tailoring and export enzymes. Interestingly, several genes show strong homology to the pfa cluster of genes involved in the biosynthesis of long chain polyunsaturated fatty acids in marine bacteria. We postulate that a mixed FAS/PKS and a hybrid NRPS/PKS assembly line each synthesize parts of the backbone that are linked together post-assembly in the case of zeamine and zeamine I. This interaction reflects a unique interplay between secondary lipid and secondary metabolite biosynthesis. Most likely, the zeamine antibiotics are produced as prodrugs that undergo activation in which a nonribosomal peptide sequence is cleaved off.
URI: 
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry
Division of Gene Technology
Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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