Title: Fluorescent oxygen sensitive microbead incorporation for measuring oxygen tension in cell aggregates
Authors: Lambrechts, Dennis ×
Roeffaers, Maarten
Kerckhofs, Greet
Roberts, Scott
Hofkens, Johan
Van de Putte, Tom
Van Oosterwyck, Hans
Schrooten, Jan #
Issue Date: 2013
Publisher: IPC Science and Technology Press
Series Title: Biomaterials vol:34 issue:4 pages:922-929
Abstract: Molecular oxygen is a main regulator of various cell functions. Imaging methods designed as screening tools for fast, in situ, 3D and non-interfering measurement of oxygen tension in the cellular microenvironment would serve great purpose in identifying and monitoring this vital and pivotal signalling molecule. We describe the use of dual luminophore oxygen sensitive microbeads to measure absolute oxygen concentrations in cellular aggregates. Stable microbead integration, a prerequisite for their practical application, was ensured by a site-specific delivery method that is based on the interactions between streptavidin and biotin. The spatial stability introduced by this method allowed for long term measurements of oxygen tension without interfering with the cell aggregation process. By making multiple calibration experiments we further demonstrated the potential of these sensors to measure local oxygen tension in optically dense cellular environments.
ISSN: 0142-9612
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Surface and Interface Engineered Materials
Centre for Surface Chemistry and Catalysis
Structural Composites and Alloys, Integrity and Nondestructive Testing
Embryo and Stemcells (-)
Molecular Imaging and Photonics
Biomechanics Section
× corresponding author
# (joint) last author

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