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Title: In vitro interactions between probiotic bacteria and milk proteins probed by atomic force microscopy
Authors: Burgain, J.
Gaiani, C. ×
Francius, G.
Revol-Junelles, A.M.
Cailliez-Grimal, C.
Lebeer, Sarah
Tytgat, Hanne
Vanderleyden, Jos
Scher, J. #
Issue Date: 2013
Publisher: Elsevier
Series Title: Colloids and Surfaces B, Biointerfaces vol:104 pages:153-162
Abstract: Interactions between microbial cells and milk proteins are important for cell location into dairy matrices. In this study, interactions between two probiotic strains, Lactobacillus rhamnosus GG and Lactobacillus rhamnosus GR-1, and milk proteins (micellar casein, native and denatured whey proteins) were studied. The bacterial surface characterization was realised with X-ray Photoelectron Spectroscopy (XPS) to evaluate surface composition (in terms of proteins, polysaccharides and lipid-like compounds) and electrophoretic mobility that provide information on surface charge of both bacteria and proteins along the 3-7 pH range. In addition, Atomic Force Microscopy (AFM) enabled the identification of specific interactions between bacteria and whey proteins, in contrast to the observed nonspecific interactions with micellar casein. These specific events appeared to be more important for the GG strain than for the GR-1 strain, showing that matrix interaction is strain-specific. Furthermore, our study highlighted that in addition to the nature of the strains, many other factors influence the bacterial interaction with dairy matrix including the nature of the proteins and the pH of the media.
ISSN: 0927-7765
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Microbial and Molecular Systems - miscellaneous
Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

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