Title: Normal ciliary beat frequency after ciliogenesis in nasal epithelial cells cultured sequentially as monolayer and in suspension
Authors: Jorissen, Mark ×
Bessems, A #
Issue Date: 19-Jun-1995
Series Title: Acta oto-laryngologica vol:115 issue:1 pages:66-70
Abstract: Ciliary beat frequency (CBF) was measured at room temperature in nasal epithelial cells from 10 control individuals at multiple time points during sequential monolayer-suspension culture. CBF was 8.4 +/- 1.6 Hz in the biopsy and decreased in monolayer culture to approximately 6 Hz. During monolayer culture the interindividual variability was increased (3.0 Hz), but not the intercellular and intracellular variability. Immediately after ciliogenesis in suspension culture, CBF was approximately 8 Hz, with a low interindividual variability. After 3 weeks in suspension culture CBF was 8.6 +/- 0.9 Hz. There was no difference in intercellular and intracellular variability of CBF in the different culture conditions. Ciliogenesis in the sequential monolayer-suspension culture results in quantitative normal ciliary activity, with low variability. The most important parameter of ciliary activity that changes during ciliary deterioration and ciliogenesis is the CBF itself.
ISSN: 0001-6489
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Research Group Experimental Oto-rhino-laryngology
× corresponding author
# (joint) last author

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