Neurourology and Urodynamics vol:31 issue:6 edition:2012 pages:742-743
Annual meeting of teh International Continence Society edition:42 location:Bejing, China date:15-19 October 2012
Hypothesis / aims of study
TRPV1 and TRPV4 are known to be expressed in the urinary bladder. TRPV1 is present both in the urothelium and in the urinary bladder nerve fibres. TRPV4 is present in the urothelium, being the two receptors co-expressed in 20% of rat urothelial cells. Recent findings indicate that TRPV4 may also be expressed in bladder afferents. In L6 dorsal root ganglia (DRG) neurons, about 30% of the entire population co-express TRPV1 and TRPV4. However, it is unknown if that neuronal population project to the urinary bladder or if remains constant upon inflammation.
During physiological conditions, TRPV1 does not seem to have a role in the urinary bladder micturition reflex. And although experiments performed with TRPV4 knockout mice indicated that TRPV4 has a role in the control of normal micturition reflex, the application of TRPV4 antagonist into the bladder of control animals did not alter bladder activity. However, during inflammatory conditions, the application of TRPV1 or TRPV4 antagonist has able to reduce of blockade cystitis-induced bladder hyperreflexia.
Therefore, our goals were to study the expression of TRPV1-TRPV4 in L6-S1 DRG population that projects to the urinary bladder, during cystitis, and to study inflamed urinary bladder reflex activity after co-application of TRPV1 and TRPV4 antagonists.