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Title: Inhibition of norovirus replication by the nucleoside analogue 2'-C-methylcytidine
Authors: Rocha-Pereira, J ×
Jochmans, Dirk
Dallmeier, Kai
Leyssen, Pieter
Cunha, R
Costa, I
Nascimento, M S J
Neyts, Johan #
Issue Date: Nov-2012
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:427 issue:4 pages:796-800
Article number: 10.1016/j.bbrc.2012.10.003
Abstract: We here report on the activity of 2'-C-methylcytidine (2CMC) [a nucleoside polymerase inhibitor of the hepatitis C virus (HCV)] on the in vitro replication of (murine) norovirus (MNV). 2CMC inhibits (i) virus-induced CPE formation, (ii) viral RNA synthesis and (iii) infectious progeny formation with EC(50) values of ∼2μM. 2CMC acts at a time-point that coincides with the onset of viral RNA synthesis. Even following 30 passages of selective pressure no MNV-resistant virus was selected, which is in line with the high barrier to resistance of the nucleoside analogue for HCV. When combined with the broad-spectrum RNA virus inhibitor ribavirin, a marked antagonistic activity was observed indicating that these molecules should not be combined for the treatment of norovirus infections. Our results suggest that 2'-C-methyl nucleoside analogues should be further explored for the treatment and prophylaxis of norovirus infections.
URI: 
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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