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Title: Endothelial basement membrane limits tip cell formation by inducing Dll4/Notch signalling in vivo
Authors: Stenzel, Denise ×
Franco, Claudio A
Estrach, Soline
Mettouchi, Amel
Sauvaget, Dominique
Rosewell, Ian
Schertel, Andreas
Armer, Hannah
Domogatskaya, Anna
Rodin, Sergey
Tryggvason, Karl
Collinson, Lucy
Sorokin, Lydia
Gerhardt, Holger #
Issue Date: Nov-2011
Publisher: Published for EMBO by Oxford University Press
Series Title: EMBO Reports vol:12 issue:11 pages:1135-43
Article number: 10.1038/embor.2011.194
Abstract: How individual components of the vascular basement membrane influence endothelial cell behaviour remains unclear. Here we show that laminin α4 (Lama4) regulates tip cell numbers and vascular density by inducing endothelial Dll4/Notch signalling in vivo. Lama4 deficiency leads to reduced Dll4 expression, excessive filopodia and tip cell formation in the mouse retina, phenocopying the effects of Dll4/Notch inhibition. Lama4-mediated Dll4 expression requires a combination of integrins in vitro and integrin β1 in vivo. We conclude that appropriate laminin/integrin-induced signalling is necessary to induce physiologically functional levels of Dll4 expression and regulate branching frequency during sprouting angiogenesis in vivo.
URI: 
ISSN: 1469-221X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Vascular Patterning Laboratory (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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