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Title: Host RNA polymerase inhibitors encoded by ϕKMV-like phages of pseudomonas
Authors: Klimuk, Evgeny
Akulenko, Natalia
Makarova, Kira S
Ceyssens, Pieter-Jan
Volchenkov, Ivan
Lavigne, Rob
Severinov, Konstantin # ×
Issue Date: Feb-2013
Publisher: Academic Press
Series Title: Virology vol:436 issue:1 pages:67-74
Article number: S0042-6822(12)00529-6
Abstract: Escherichia coli bacteriophage T7 is a founding member of a large clade of podoviruses encoding a single-subunit RNA polymerase (RNAP). Phages of the family rely on host RNAP for transcription of early viral genes; viral RNAP transcribes non-early viral genes. T7 and its close relatives encode an inhibitor of host RNAP, the gp2 protein. Gp2 is essential for phage development and ensures that host RNAP does not interfere with viral RNAP transcription at late stages of infection. Here, we identify host RNAP inhibitors encoded by a subset of T7 clade phages related to ϕKMV phage of Pseudomonas aeruginosa. We demonstrate that these proteins are functionally identical to T7 gp2 in vivo and in vitro. The ability of some Pseudomonas phage gp2-like proteins to inhibit RNAP is modulated by N-terminal domains, which are absent from the T7 phage homolog. This finding indicates that Pseudomonas phages may use external or internal cues to initiate inhibition of host RNAP transcription and that gp2-like proteins from these phages may be receptors of these cues.
URI: 
ISSN: 0042-6822
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
× corresponding author
# (joint) last author

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