Title: Structural recognition mechanisms between human Src homology domain 3 (SH3) and ALG-2-interacting protein X (Alix)
Authors: Shi, Xiaoli
Betzi, Stephane
Lugari, Adrien
Opi, Sandrine
Restouin, Audrey
Parrot, Isabelle
Martinez, Jean
Zimmermann, Pascale
Lecine, Patrick
Huang, Mingdong
Arold, Stefan T
Collette, Yves
Morelli, Xavier # ×
Issue Date: Jun-2012
Publisher: Elsevier on behalf of the Federation of European Biochemical Societies
Series Title: FEBS Letters vol:586 issue:13 pages:1759-1764
Abstract: The functions of Src family kinases are tightly regulated through Src homology (SH) domain-mediated protein-protein interactions. We previously reported the biophysical characteristics of the apoptosis-linked gene 2-interacting protein X (Alix) in complex with the haemopoietic cell kinase (Hck) SH3 domain. In the current study, we have combined ITC, NMR, SAXS and molecular modeling to determine a 3D model of the complex. We demonstrate that Hck SH3 recognizes an extended linear proline-rich region of Alix. This particular binding mode enables Hck SH3 to sense a specific non-canonical residue situated in the SH3 RT-loop of the kinase. The resulting model helps clarify the mechanistic insights of Alix-Hck interaction.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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