Title: Assessment of the microbiota in microdissected tissues of Crohn's disease patients
Authors: De Hertogh, Gert ×
Lemmens, Bart
Verhasselt, Peter
de Hoogt, Ronald
Sagaert, Xavier
Joossens, Marie
Van Assche, Gert
Rutgeerts, Paul
Vermeire, Severine
Aerssens, Jeroen #
Issue Date: 2012
Publisher: Hindawi
Series Title: International Journal of Inflammation vol:2012 issue:? pages:505674-?
Abstract: The microbiota of the gastrointestinal tract is frequently mentioned as one of the key players in the etiopathogenesis of Crohn's disease (CD). Four hypotheses have been suggested: the single, still unknown bacterial pathogen, an abnormal overall composition of the bowel microbiota ("dysbiosis"), an abnormal immunological reaction to an essentially normally composed microbiota, and increased bacterial translocation. We propose that laser capture microdissection of selected microscopic structures, followed by broad-range 16S rRNA gene sequencing, is an excellent method to assess spatiotemporal alterations in the composition of the bowel microbiota in CD. Using this approach, we demonstrated significant changes of the composition, abundance, and location of the gut microbiome in this disease. Some of these abnormal findings persisted even after macroscopic mucosal healing. Further investigations along these lines may lead to a better understanding of the possible involvement of the bowel bacteria in the development of clinical Crohn's disease.
ISSN: 2042-0099
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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