Phylogeography of dengue virus serotype 4, Brazil, 2010-2011
Nunes, Marcio Roberto Teixeira Faria, Nuno Rodrigues × Vasconcelos, Helena Baldez Medeiros, Daniele Barbosa de Almeida Silva de Lima, Clayton Pereira Carvalho, Valéria Lima Pinto da Silva, Eliana Vieira Cardoso, Jedson Ferreira Sousa, Edivaldo Costa Nunes, Keley Nascimento Barbosa Rodrigues, Sueli Guerreiro Abecasis, Ana Barroso Suchard, Marc A Lemey, Philippe Vasconcelos, Pedro Fernando da Costa #
National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC)
Dengue virus serotype 4 (DENV-4) reemerged in Roraima State, Brazil, 28 years after it was last detected in the country in 1982. To study the origin and evolution of this reemergence, full-length sequences were obtained for 16 DENV-4 isolates from northern (Roraima, Amazonas, Pará States) and northeastern (Bahia State) Brazil during the 2010 and 2011 dengue virus seasons and for an isolate from the 1982 epidemic in Roraima. Spatiotemporal dynamics of DENV-4 introductions in Brazil were applied to envelope genes and full genomes by using Bayesian phylogeographic analyses. An introduction of genotype I into Brazil from Southeast Asia was confirmed, and full genome phylogeographic analyses revealed multiple introductions of DENV-4 genotype II in Brazil, providing evidence for >3 introductions of this genotype within the last decade: 2 from Venezuela to Roraima and 1 from Colombia to Amazonas. The phylogeographic analysis of full genome data has demonstrated the origins of DENV-4 throughout Brazil.