Title: Periostin as a biomarker of the amniotic membrane
Authors: Dobreva, Mariya ×
Lhoest, Larissa
Pereira, Paulo N G
Umans, Lieve
Chuva de Sousa Lopes, Susana M
Zwijsen, An #
Issue Date: 1-Jul-2012
Publisher: Hindawi Publishing Corporation
Series Title: Stem Cells International vol:2012 issue:2012
Article number: 987185
Abstract: Tracing the precise developmental origin of amnion and amnion-derived stem cells is still challenging and depends chiefly on analyzing powerful genetic model amniotes like mouse. Profound understanding of the fundamental differences in amnion development in both the disc-shaped primate and human embryo and the cup-shaped mouse embryo is pivotal in particular when sampling amniotic membrane from nonprimate species for isolating candidate amniotic stem cells. The availability of molecular marker genes that are specifically expressed in the amniotic membrane and not in other extraembryonic membranes would be instrumental to validate unequivocally the starting material under investigation. So far such amniotic markers have not been reported. We postulated that bone morphogenetic protein (BMP) target genes are putative amniotic membrane markers mainly because deficiency in one of several components of the BMP signaling cascade in mice has been documented to result in defective development of the early amnion. Comparative gene expression analysis of acknowledged target genes for BMP in different extraembryonic tissues, combined with in situ hybridization, identified Periostin (Postn) mRNA enrichment in amnion throughout gestation. In addition, we identify and propose a combination of markers as transcriptional signature for the different extraembryonic tissues in mouse.
ISSN: 1687-966X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Developmental Signaling
Embryo and Stemcells (-)
× corresponding author
# (joint) last author

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