Author:

Keywords:

Y-chromosome, bison, wisent, banteng, Madura cattle, Bali cattle, Science & Technology, Life Sciences & Biomedicine, Ecology, Evolutionary Biology, Genetics & Heredity, Environmental Sciences & Ecology, SATELLITE DNA, Y-CHROMOSOME, GEOGRAPHIC-DISTRIBUTION, BISON POPULATIONS, DOMESTIC CATTLE, MITOCHONDRIAL, AFRICAN, INTROGRESSION, MTDNA, AFLP, Animals, Bison, Cattle, DNA Primers, Electrophoresis, Agar Gel, Genetic Markers, Genetics, Population, Hybridization, Genetic, Inheritance Patterns, Male, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Y Chromosome, 0603 Evolutionary Biology, 0604 Genetics, 3104 Evolutionary biology, 3105 Genetics

Abstract:

The genetic integrity of crossfertile bovine- or cattle-like species may be endangered by species hybridization. Previously, amplified fragment length polymorphism, satellite fragment length polymorphism and microsatellite assays have been used to analyze the species composition of nuclear DNA in taurine cattle, zebu, banteng and bison populations, while mitochondrial DNA reveals the origin of the maternal lineages. Here, we describe species-specific markers of the paternally transmitted Y-chromosome for the direct detection of male-mediated introgression. Convenient PCR-restriction fragment length polymorphism and competitive PCR assays are shown to differentiate the Y-chromosomes of taurine cattle, American bison and European bison, and to detect the banteng origin of Indonesian Madura and Bali cattle bulls.