Standardisation of EGFR FISH in colorectal cancer: results of an international interlaboratory reproducibility ring study
Sartore-Bianchi, Andrea × Fieuws, Steffen Veronese, Silvio Moroni, Mauro Personeni, Nicola Frattini, Milo Torri, Valter Cappuzzo, Federico Vander Borght, Sara Martin, Vittoria Skokan, Margaret Santoro, Armando Gambacorta, Marcello Tejpar, Sabine Varella-Garcia, Marileila Siena, Salvatore #
British Medical Journal Publishing Group
Journal of Clinical Pathology vol:65 issue:3 pages:218-223
Epidermal growth factor receptor (EGFR) gene copy number evaluated by fluorescence in situ hybridisation (FISH) can discriminate among KRAS wild-type patients those with better outcome to EGFR-targeted therapy in metastatic colorectal cancer, further enhancing selection of patients. Nevertheless, enumeration of gene copies is challenging and the lack of analytical standardisation has limited incorporation of the test into the clinical practice. We therefore assessed EGFR FISH interlaboratory consensus among five molecular diagnostic reference centres.